What are the steps of IVF?
Fertility Clinic is a State of the art accredited by the Department of health. We offer many technologies of assisted reproductive technology (ART) to help couples achieve a successful pregnancy. One of the most popular procedures is in vitro fertilization (IVF). More information about the steps of the IVF procedure.
Step 1: Ovarian (COH) controlled hyperstimulation
The first step of the in vitro fertilization is controlled ovarian hyperstimulation. COH is achieved using different methods. The most common method is the long protocol of GnRH (Lupron) agonist. This involves the secretion of the gonadotropin suppression to prevent premature ovulation. Once achieved the suppression, the woman receives daily injections of gonadotrophins. Hormonal evaluations and ultrasound imaging is used to control the follicular development. When the follicles have reached the right size, is administered human chorionic gonadotropin (hCG), and egg retrieval is performed within 34 hours of the injection.
Step 2: Recovery of egg
Egg retrieval is performed in a room with intravenous sedation procedure. In guided transvaginal ultrasound ovarian follicles are sucked. Once extracted using a small needle, follicular fluids are analyzed by the embryologist to locate the available ovules. After recovery, the eggs are placed in a special medial and cultivated using an incubator.
Step 3: fertilization
Normal, mobile sperm are transferred to a dish containing eggs (a process called standard insemination). If the parameters of sperm are not normal, the ICSI technique, which involves injecting the egg with a sperm with a high-powered microscope is used. The embryologist selects a sperm with a microneedle and injected directly into the cytoplasm of the egg. This increases the likelihood of fertilization. Fertilization will occur within 18 hours after insemination.
Step 4: Embryo culture
Now fertilized eggs are zygotes, and are grown in a special formulated medium to support growth. In the second or third day after egg retrieval, the zygotes are evaluated to see if the growth and development are normal. Blastocyst culture gives the embryo implantation potential. To reduce the chance of multiple pregnancies, fewer embryos are transferred. Embryo transfer is recommended three days for cycles with low numbers or poor quality egg.
Step 5: Embryo quality
To assess the quality of an embryo, are several criteria to follow. This is crucial to decide which embryos are suitable for the transfer process. On the day of the scheduled transfer, the embryologist photography and evaluates the embryos. Embryologist and fertility specialist decide what transfer based on the appearance and the pace of development. In general, when the embryos are transferred the division (three days after egg retrieval) Stadium, or in the blastocyst stage (day five following egg retrieval). The laboratory uses a rating system to evaluate the quality of the embryo.
Step 6: Embryo transfer
When embryos are at the stage of division, they have around 4-8 cells. The embryologist examines the number of cells and evaluated by symmetry. Fragmentation occurs when cells divide, which results in overcrowding. The laboratory prefers that no fragmentation because of this there is. Day 5 embryos are blastocysts, which increase in size and more developed. Most widespread is the embryo, better will be the quality. With transfer of the embryo, embryos are loaded into a catheter, small, soft and then placed into the uterus through the cervix.