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Preimplantation Genetic Testing in Dubai

Preimplantation genetic testing, also called preimplantation genetic diagnosis (PGD), is a process of removing a cell from an embryo for genetic testing. This is done at PRC Dubai fertility center to assess for diseases and conditions that could interfere with the embryo’s survival once it is introduced into the uterus. PGD is used in the in vitro fertilization (IVF) process.

History of PGD

The first IVF procedure was completed in 1978. Years later, scientists began testing the embryo for potential health problems as well as chromosomal abnormalities. PGD was used in humans in 1990, and many improvements have occurred with PGD in the last 25 years. Genetic technologies used on cells removed from embryos and embryo biopsy have improved drastically over the last couple of decades.

Stages of Egg and Embryo Development

Three basic stages exist at which embryos or eggs may be biopsied to remove cells for testing. These include:

  • Polar body – This is an early stage where the polar body of the egg is removed and tested. A mature human oocyte (egg) has one polar body, and a fertilized egg has two polar bodies. This technique does not alter the egg or embryo so that it cannot implant into the uterus or develop into a fetus. However, there is the chance of damage to the developing embryo with polar body biopsy.
  • Cleavage stage – Day three of the embryo’s development is stage three. At this point, the embryo has 6-10 cells. Removal of the outer shell (zona pellucida) is performed so 1-2 cells can be removed for testing. Stage three development is important for the embryo’s development, so many IVF programs are not performing this technique.
  • Blastocyst stage – The trophectoderm biopsy is the technique performed during the blastocyst stage of embryo development. After the embryo is formed into a multi-cell mass (trophectoderm), it has a fluid-filled cavity. This biopsy involves making a small hole in the shell of the embryo so several cells can be removed for testing.

PGD Clinics

The best fertility clinics for PGD are the ones with the best IVF success rates. The clinic should have an excellent culture system in order to obtain the maximum number of quality cells for biopsy. The clinic should also be skilled in embryo biopsy and blastocyst freezing, as well as successful in thawing frozen eggs and embryos.

Candidates for PGD

The most common reason for preimplantation genetic testing is advanced age. Women of advancing age have increased rates of chromosomally abnormal eggs, which develop into abnormal embryos. Five main types of clients should use PGD. These include clients who:

  • Are having IVF with female age of 38 years or older
  • Have repeated IVF failure, defined as three or more failed attempts
  • Have a family history of inherited genetic diseases
  • Are carriers of chromosomal translocations
  • Have had recurrent miscarriages

Rates of Chromosomal Abnormality

The percentage of eggs with some type of chromosomal abnormality does increase as the woman ages. Approximately 45% of human embryos have some form of chromosomal abnormality. By testing the embryos available for transfer, we can avoid use of ones with abnormalities and choose healthy, high-quality embryos for transfer the woman’s uterus. PGD testing of early embryos will increase the chances of a successful pregnancy.

Technology used to Assess Chromosomes

There are three technologies currently being used for assessment of chromosomal abnormalities. These are:

  • Array Comparative Genomic Hybridizaiton (aCGH)
  • Quantitative Real-Time Polymerase Chain Reaction (qPCR)
  • Single Nucleotide Polymorphism Microarrays (SNP)

PRC Dubai is the top fertility center in Dubai. After two successful decades of treatment at several metro Los Angeles fertility centers, PRC is now open in Dubai Healthcare City! Call us today at 800-4544.

Here is a video on PGD Testing:

 

The Basics of Egg Freezing in Dubai

Egg Freezing in Dubai Healthcare City

Women often delay childbearing due to personal circumstances. Because egg quality declines with age, women now have the option of egg freezing so the eggs can be fertilized and implanted at a later date. This form of assisted reproductive technology enables women to cryopreserve (freeze) eggs when they are at their healthiest.

The Natural Egg Reserve

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On average, a woman has around 600,000 eggs, which are present at birth. This supply diminishes at a rate of 1,000 per month throughout the lifespan. This egg reserve decline is often referred to as a woman’s biological clock. The loss of eggs continues, regardless of pregnancy, absence of menses, or if the woman is taking oral contraceptives. The rate of egg loss accelerates when a woman is in her late 30s and early 40s. By midlife (43-47 years of age), a woman usually loses her natural ability to have children. What is more important, egg quality diminishes with time, and chromosome defects and miscarriages are more common with a later age of pregnancy.

Preparing for Egg Freezing

Women interested in freezing eggs will first undergo a series of fertility tests to assess reproductive potential and readiness for IVF. This involves:

  • Initial evaluation of ovarian reserve – The consultation with the fertility specialist involves taking a medical history, conducting a physical examination, and assessing the rate of future success. Testing include assessing follicle-stimulating hormone (FSH) levels, estrogen levels, antral follicle count, and anti-Mullerian hormone (AMH) levels.
  • Egg retrieval process – This process involves taking fertility medications to stimulate follicle growth so multiple eggs are produced. Ultrasound and blood levels are used to monitor progress, and eggs are retrieved during a simple office procedure.
  • Egg preservation – The retrieved eggs are then processed through a rapid freezing process called vitrification. The frozen eggs are cryopreserved until they are needed. It is important for the client to maintain contact with the fertility clinic so the eggs will not be abandoned.

Creating Embryos using Frozen Eggs

The process of using frozen eggs involves:

  • Thawing – When the client decides to have a child, the eggs can be thawed in the laboratory. Thawing occurs on the day the eggs are to be fertilized and should be synchronized with the woman’s cycle. Around 90% of the frozen eggs survive the freeze-to-thaw process.xxxAccordion2

 

  • Fertilization – Thawed eggs are then fertilized in our laboratory using intracytoplasmic sperm injection (ICSI), where a single sperm is strategically placed into each egg. ICSI is required for fertilization because the protein coat around the egg becomes hardened through the freezing process. Around 70% of the injected eggs are expected to become fertilized.

 

  • Embryo culture – After fertilization, the eggs remain in the laboratory for three to five days to develop into embryos. While not all develop into embryos, the number of eggs that survive and progress is related to the woman’s age at the time of egg freezing.

 

  • Embryo transfer – The number of embryos transferred to the client’s uterus depends on her age at the time of egg freezing. Younger women have greater likelihood of implantation, so fewer embryos are transferred. The transfer is done using a tiny insertion catheter and ultrasound guidance.

PRC Dubai offers first rate fertility treatment in Dubai Healthcare City. After 25 years of success in the US, PRC is now offering state-of-the-art options in the Middle East! Here’s a video on Egg Freezing:

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